mouse monoclonal anti spectrin 3a9  (Developmental Studies Hybridoma Bank)

Journal: Biomolecules

Article Title: The Memory Gene, Murashka, Is a Regulator of Notch Signalling and Controls the Size of the Drosophila Germline Stem Cell Niche

doi: 10.3390/biom15081082

Figure Lengend Snippet: P-element insertion in mura results in an increase in the number of cap cells: ( A ) The mura gene locus (adapted from Flybase http://flybase.org/ accessed on 22 July 2025). Indicated are the positions of the P-element insertions used in this study. Orange rectangles indicate protein coding sequences. The dashed line indicates the deficiency used, Df(3R)BSC476. ( B ) Schematic representation of the structure of the niche. ( C , D ) Wild-type (WT) and mura EY03448 germarium stained with anti-α-Spectrin, which marks the spherical spectrosomes of the GSCs (green—spherical structures indicated by the asterisks). Anti-LaminC (green—nuclear membranes) and anti-Coracle (purple) mark the cap cells. ( E ) PCR using primers muraF1 and R1 to confirm the precise excision of EY03448 P-element. A 720 bp band is observed when the P-element is absent. In flies where the P-element has been remobilised, this WT band is observed. ( F ) Scoring of niche size. Mean cap cell numbers are significantly different between WT and mura EY03448 , but the phenotype is restored to WT following excision of the P-element. Error bars represent SEM, and * indicates p < 0.05 compared to WT by ANOVA with Tukey HSD test.

Article Snippet: The primary antibodies were Mouse alpha-Spectrin (DSHB 1/20), Mouse LaminC DSHB 1/10) and guinea pig Coracle (Richard Fehon, University Chicago, IL, USA 1/10,000).

Techniques: Staining

Journal: Biomolecules

Article Title: The Memory Gene, Murashka, Is a Regulator of Notch Signalling and Controls the Size of the Drosophila Germline Stem Cell Niche

doi: 10.3390/biom15081082

Figure Lengend Snippet: Loss and gain of function of mura are associated with the misregulation of niche size: ( A , B ) Complementation analysis of mura alleles. Germaria were stained with anti-Coracle (purple) to mark cap cells and anti-α-Spectrin to mark germ line stem cells and DAPI (greyscale) for nuclei. ( A ) depicts different z sections through the niche of a GSV3176/EY03447 germarium, showing an expanded niche comprising two clusters of cap cells on either side of the germarium. ( B ) Quantification of mean cap cell numbers across the different genotypes as indicated. * indicates p < 0.05 compared to WT, by ANOVA and Tukey HSD test. ( C , E ) Expression of the mura RNAi in the developing niche increases niche size. * indicates p < 0.05 compared to UAS-muraRNAi without Gal4, by t -test. ( F ) Expression of mura cDNA by C587 Gal4 reduced the size of the niche compared to the Gal4 driver alone. * indicates p < 0.05 compared to Gal4 driver alone by t -test. ( D , G ) Expression of mura cDNA rescues the mura mutant niche phenotype. * indicates p < 0.05 compared to C587Gal4; KG04118/Df(476) and UAS Mura; KG04118/Df(476), by ANOVA with Tukey HSD test. Error bars in ( B , E , F ) are SEM. WT data is also shown in F.

Article Snippet: The primary antibodies were Mouse alpha-Spectrin (DSHB 1/20), Mouse LaminC DSHB 1/10) and guinea pig Coracle (Richard Fehon, University Chicago, IL, USA 1/10,000).

Techniques: Staining, Expressing, Mutagenesis